Venepuncture

Checklist

Preparation Wash – Name – Explain
Ensure patient and test request details match
Check for allergies
Position patient appropriately for procedure
Clean preparation surface
Gather equipment Clean equipment tray
Wash hands
Tourniquet
Skin cleaning equipment
Needle
Blood bottle(s)
Gauze
Plaster
Safe sharps disposal
Site identification & cleaning Return to patient
Wash hands
Select appropriate site
Place tourniquet
Identify appropriate vein
Clean skin
Allow skin to dry
Venepuncture Wash hands and put on gloves
Insert needle into vein
Attach & fill blood bottle(s) according to order of draw
Release tourniquet
Cover site with gauze
Withdraw needle
Apply pressure to gauze
Dispose of sharps safely
Ensure haemostasis
Cover wound
Finishing Label bottles at bedside
Dispose of all waste appropriately
Wash hands

 

Explanation

Preparation

Wash, name, explain:

  • Wash your hands
  • Introduce yourself by name and role
  • Check the patient’s name and date of birth
  • Explain the task and get consent 
  • Ensure that the patient’s details match those on the test request forms
  • Check whether the patient has any known allergies, e.g., to plasters

 

“Hello, I’m one of the doctors. I’ve been asked to take a sample of blood from you. This will involve inserting a small needle into your arm. It may be slightly uncomfortable but should not be painful. You can ask me to stop at any time. Would that be okay?”

Position the patient appropriately for the procedure. For venepuncture, ensure the patient is sitting in a comfortable position, able to fully extend their arm at the elbow, and you are able to comfortably access the procedure site. You may wish to place a pillow under the patient’s arm for support.

Clean your preparation space, typically a clinical trolley, using appropriate surface cleaning agents.

 

Gather Equipment

Clean an equipment tray and place it on your preparation surface. Wash your hands again after cleaning surfaces.

Gather the following equipment and place it in your clean tray, ensuring that key parts remain protected:

  • Tourniquet
  • Skin cleaning apparatus – usually alcohol-based
  • Needle – the type used will depend on your local venepuncture protocol
  • Blood bottle(s) – the type of bottles, colour coding and order of draw will vary according to your local protocol
  • Gauze
  • Plaster
  • Safe sharps disposal e.g., sharps bin

 

Site Identification & Cleaning

Return to the patient, wash your hands, and confirm that they are still happy to proceed. Ask the patient if they have a preference which arm you take the blood sample from. Ensure that there are no contraindications to using an arm e.g., active or recent infusion, fistula, lymphoedema. Identify the best site on the chosen arm, usually this is the antecubital fossa.

Attach the tourniquet proximal to the preferred site. Look and feel for an appropriate vein. The best veins will be palpable,  bouncy feeling, straight, and not too close to a venous junction.

Clean the skin using the appropriate equipment for at least 30 seconds and then allow the site to air dry for a further 30 seconds.

 

Venepuncture

Wash your hands and put on your gloves. Do not touch the venepuncture site you have cleaned.

Unsheathe the venepuncture needle. Using your nondominant hand, anchor the skin just distal to the venepuncture site to stabilise the vein and minimise movement. Insert the needle into the vein at an angle of approximately 30° to the skin.

Keeping the needle as still as possible, attach the blood bottle to the needle. Once the bottle is adequately filled, detach it and repeat for any additional bottles required, following your local order of draw policy.

Once you have taken the required samples, release the tourniquet while keeping the needle as still as possible. Place gauze over the needle insertion site, remove the needle and immediately dispose of it in your sharps disposal. Apply firm pressure to the venepuncture site using the gauze.

Ensure pressure is applied until the site has stopped bleeding. This may take longer if the patient is taking anticoagulants. Once haemostasis has been established, cover the site with a plaster.

 

Finishing

Confirm that the patient is feeling well. 

Ensure you label the blood bottles at the bedside, either handwriting the labels on the bottles or applying preprinted stickers depending on your local procedure. 

Dispose of all waste appropriately and clean your tray and trolley. Wash your hands.

 

Last updated Mar 2025

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